Composite

Part:BBa_K1149001:Design

Designed by: Margarita Kopniczky   Group: iGEM13_Imperial_College   (2013-09-09)


protein secretion in E.coli


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 378
    Illegal SpeI site found at 1010
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 378
    Illegal SpeI site found at 1010
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 122
    Illegal BamHI site found at 221
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 378
    Illegal SpeI site found at 1010
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 378
    Illegal SpeI site found at 1010
    Illegal NgoMIV site found at 1053
    Illegal NgoMIV site found at 2180
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

E.coli optimized


Source

bacterial

References